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. 2003 Dec;71(12):7069–7078. doi: 10.1128/IAI.71.12.7069-7078.2003

FIG. 4.

FIG. 4.

(A) The tir deletion strain CVD463 (Δtir EPEC) has no impact on TER. Uninfected T84 monolayers and those uninfected with wild-type EPEC or EPEC Δtir were serially assessed for TER. As shown, expression of Tir is required for the EPEC-associated decrease in TER. The data shown represent the mean ± SEM (n = 8; P = 0.003 for EPEC versus Δtir EPEC) from three experiments with duplicate or triplicate samples. (B) Deletion of tir also blocks redistribution of β1-integrin. Uninfected T84 monolayers or those infected with EPEC Δtir or wild-type EPEC for 6 h were selectively labeled with biotin on the apical (AP) or basolateral (BL) surface, immunoprecipitated with β1-integrin antibody, and detected with alkaline phosphatase-streptavidin. Even after 6 h of infection, no redistribution of β1-integrin was detected in the monolayers infected with EPEC Δtir (EPEC Δtir versus wild-type EPEC after 6 h [n = 3], P = 0.02). Densitometry data are presented as percentages of the total for the apical and basolateral surfaces. The immunoblot is representative of those from three separate experiments.