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Primers used in this study
| Target | Forward primer | Reverse primer | MgCl2 (mM) | Melting temp (°C) | Product size (bp) |
|---|---|---|---|---|---|
| PBGDa | CCTGGTTGTTCACTCCCTGA | CAACAGCATCACAAGGGTTTT | 3 | 84.4 | 98 |
| IL-4 | TTTTGAACGAGGTCACAGGA | AGCCCTACAGACGAGCTCAC | 3 | 86.9 | 107 |
| IFN-γ | CTTCTTCAGCAACAGCAAGG | TGAGCTCATTGAATGCTTGG | 3 | 84.7 | 101 |
PBGD, porphobilinogen deaminase.
