FIG. 1.

Genetic organization of the Hif capsule locus and adjacent DNA. Arrows indicate genes and ORFs. Region I contains four genes, on the opposite strand, homologous to those found in Hia and Hib, bexDCBA (left-hatched arrows). Region II is comprised of three serotype-specific genes designated fcs1, fcs2, and fcs3 (black arrows). Region III has two genes, hcsA and hcsB, also found in the cap locus of Hib (right-hatched arrows). Six additional ORFs outside of the Hif cap locus are noted with white arrows. Vertical lines show cleavage sites for restriction endonucleases EcoRI (E) and PstI (P). Horizontal lines below the restriction map show the locations and sizes of the probes used to identify cosmid clones with the Hif cap locus.