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. 2003 Dec;71(12):7202–7207. doi: 10.1128/IAI.71.12.7202-7207.2003

FIG. 2.

FIG. 2.

fcs1, fcs2, and fcs3 expression detected in log-phase Hif 700222 cells and shown to be cotranscribed by RT-PCR. Lane 1, 1-kb standard marker (Promega Corp., Madison, Wis.); lanes 3, 6, 9, 12, and 15, RT negative controls; lanes 4, 7, 10, 13, and 16, positive controls with chromosomal DNA from Hif 700222 as template; lane 2, Fcs1-a and Fcs1-b RT (+); lane 5, Fcs2-a and Fcs2-b RT (+); lane 8, Fcs3-a and Fcs3-b RT (+); lane 11, Fcs1-2-a and Fcs1-2-b RT (+); lane 12, Fcs1-2-a and Fcs1-2-b RT (-); lane 13, Fcs1-2-a and Fcs1-2-b as PCR control; lane 14, Fcs2-3-a and Fcs2-3-b RT (+). Primers and product sizes are listed in Table 1.