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. 2003 Dec;71(12):7202–7207. doi: 10.1128/IAI.71.12.7202-7207.2003

FIG. 3.

FIG. 3.

PCR analysis of the end junctions and outside DNA of the Hif cap locus and H. influenzae serotypes a and e. Lanes 1 and 14, 1-kb standard marker (Promega Corp.). Hif 700222 chromosomal DNA was amplified with primer pairs capfMerT and SodC 3970-2951 (lane 3), capfSodC and capfBexA (lane7), and capf region III and HI1637 (lane 11). Hia ATCC 9006 chromosomal DNA was amplified with primer pairs capfMerT and SodC 3970-2951 (lane 4), capfSodC and capfBexA (lane 8), and capf region III and HI1637 (lane 12). Hie ATCC 8142 chromosomal DNA was amplified with primer pairs capfMerT and SodC 3970-2951 (lane 5), capfSodC and capfBexA (lane 9), and capf region III and HI1637 (lane 13). Lanes 2, 6, and 10 are the no-template controls. Primers and product sizes are listed in Table 1.