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. 2003 Dec;71(12):7099–7108. doi: 10.1128/IAI.71.12.7099-7108.2003

FIG. 3.

FIG. 3.

Localization of macrophages and AFB in resected lungs. Macrophages, stained with CD68 (A, C, and E) and AFB stained with carbolfuchsin (B, D, and F) in the left upper-lobe cavity wall of the open lesion of patient 1 are shown. CD68 staining is seen at the cavity (cav) surface (A and C), within the necrotic area (nec) (A and C), and in the granulomatous area below the necrotic area (E). AFB are seen predominantly within macrophages at the cavity surface (D) and not in the Langhans cells or macrophages of the granulomatous tissue (F). The inset in panel D shows AFB in cells at the luminal surface of the right-lower-lobe cavity of patient 2. AFB are also seen in macrophages within the liquefied (liq) material adjacent to the necrotic area (H) of the left-lower-lobe fibrotic nodule undergoing breakdown (liquefaction) in patient 3. Magnifications, ×10 (A and B), ×40 (C, E, F, and G), ×80 (D, inset), and ×200 (D and H).