FIG. 4.

Analysis of Erp and OspC protein expression by B. burgdorferi grown at a constant 34°C. B31-MI bacteria were grown at 23°C to mid-late logarithmic phase, diluted 1:100 into fresh media, and shifted to 34°C. Cultures were then maintained at 34°C for three more passages. Cell lysates were prepared from bacteria grown at each passage, and Western blot analysis utilizing specific antibodies directed against ErpA/I/N (A) and OspC (B) was conducted. Each blot was then stripped and reprobed with murine anti-FlaB monoclonal antibody to ensure that equal quantities of protein were loaded in all lanes.