TABLE 1.
Bacterial strains and plasmids
| Bacterial strain or plasmid | Characteristic(s) | Source or reference |
|---|---|---|
| E. coli DH5α | F−φ80d lacZΔM15Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 λ thi-1 gyrA96 relA1 | Gibco/BRL |
| S. enterica serovar Enteritidis strains | ||
| SE2472 | Clinical isolate, mouse virulent and egg resistant | 30 |
| SE2472ΔyafD | yafD::kan derivative of SE2472 | This study |
| SE2472ΔyafE | yafE::kan derivative of SE2472 | This study |
| SE2472ΔxthA | xthA::kan derivative of SE2472 | This study |
| S. enterica serovar Typhimurium strains | ||
| ST3744 | Clinical isolate, egg albumen susceptible | This study |
| ST3744ΔyafD | yafD::kan derivative of ST3744 | This study |
| Plasmids | ||
| pBluescript II KS | Apr, high-copy-number plasmid | Stratagene |
| pKD4 | Apr, Kanr oriRγ | 8 |
| pKD46 | Apr, containing the Red recombinase of λ phage | 8 |
| pRB3-273C | Apr, low- to medium-copy-number plasmid for Salmonella | 4 |
| pK3 | Derivative of pRB3-273C containing yafD and yafE | This study |
| pRB3yafD | Derivative of pRB3-273C containing yafD | This study |
| pRB3xthA | Derivative of pRB3-273C containing xthA | This study |