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Surface labeling of GAS with Fba antibodies and FH. Fba+ (90-226 emm1::km) and Fba− GAS (DC276) were incubated with Fba antiserum (middle) followed by FITC-labeled goat anti-rabbit IgG. Bacteria for the lower panels were incubated with FH, goat anti-human FH serum, and donkey anti-rabbit IgG conjugated to rhodamine red.
