Figure 5.

PIC assembly at huγ-genes in ln2 and ln2-Ik^null yolk sac EryC. (A–C) ChIP on ln2 and ln2-Ik^null yolk sac EryC carried out with TBP, Pol II and Pol II phospho Ser2 (PCTD) antibodies; fold enrichments (y-axis) were calculated as described in Figure 1E and are plotted as the mean ± SD of the measurements; a value of 1 (dashed line) indicates no enrichment; *P ≤ 0.05 by Student’s t-test; huε, huε-promoter; huγ, huγ-promoters; huγg, huγ-genes; huβ, huβ-promoter; Gpa, Glycophorin A promoter; dark gray bars: ln2 yolk sac EryC; light gray bars: ln2-Ik^null yolk sac EryC; white bars: isotype-matched Ig (Ig ctl); (D) RT–qPCR of huγ-gene 5′- and 3′-primary transcripts performed on equal amounts of ln2 and ln2-Ik^null yolk sac EryC using random primers for cDNA synthesis; transcript quantification was calculated according to Pfaffl (73) using the huγ-globin specific primer sets (Pr) depicted as colored bars and mouse Gapdh primary transcripts as internal control; ratios are plotted as the mean ± SD of the measurements; n ≥ 3; P ≤ 0.05; Ex: indicates the three exons of the huγ-gene.