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. 2011 Jan 17;39(9):3505–3519. doi: 10.1093/nar/gkq1271

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Ikaros interacts with Cdk9 in Jurkat and COS-7 cell lines. (A) WB performed on total cell lysates prepared from Jurkat cells (Ju) or Jurkat cells stably transfected with the pOZN-FH-Ik expression vector (Ju/Ik); endogenous (Ik) as well as double Flag/HA-Ikaros-specific bands (FH-Ik) are indicated; (B) Protein co-IP of total cell lysates prepared from Ju/Ik cells; filled circles represent Ig bands; WCE: Ju/Ik total cell lysate; (C) Protein co-IP of total cell lysates prepared from MEL cells; I, Input; M20, anti-Gata-1 antibodies, clone M20; N6, anti-Gata-1 antibodies, cloneN6; Ig, isotype-matched Ig; (D and E) Protein co-IP of total cell lysates prepared from COS-7 cells transfected (Tr) with empty vector (mock, M), Flag/HA-Ikaros (Ik), Gata-1 (G1) or Flag/HA-Ikaros together with Gata-1 (G/Ik); antibodies used for protein co-IP (IP) and WB are indicated on each panel; I, Input; M20, anti-Gata-1 antibodies, clone M20; N6, anti-Gata-1 antibodies, cloneN6; filled circles represent Ig bands.