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. 2011 Jan 17;39(9):3520–3528. doi: 10.1093/nar/gkq1273

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Published by Oxford University Press 2011.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — ISW2-induced nucleosome translocation relieves the polar barrier. (A) Nucleosome positions and translocation distances on permissive (603-P and 605-P) and non-permissive (603-NP and 601-NP) templates before and after remodeling by ISW2. The PBS is indicated by a rectangle. (B) 603-NP or (C) 601-NP nucleosomes (N) or nucleosomes remodeled with ISW2 (N*ISW2), or free DNA (D) were transcribed by Pol II in 40 mM KCl without NTPs, or in 40, 150, 300 or 1000 mM KCl with 0.4 mM NTPs. Pulse-labeled RNA was analyzed by denaturing PAGE. Only the run-off products are shown (see Supplementary Figure S2 for the entire gel). (D) Quantitation of run-off transcripts in (B) and (C). The amount of run-off transcript produced in 1 M KCl was set at 100%. The data represent means of three independent experiments, with standard errors.