Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jan 17;39(9):3520–3528. doi: 10.1093/nar/gkq1273

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published by Oxford University Press 2011.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Analysis of ISW2-induced nucleosome translocation. (A) Nucleosomes were reconstituted on 210- or 300-bp end-labeled DNA fragments containing the 601, 603 or 605 nucleosome positioning sequences (Figure 1A), incubated with ISW2 and analyzed by native PAGE. Nucleosome positions are indicated. M, end-labeled pBR322-MspI digest. (B) Mapping of 603-P nucleosome positions: restriction enzyme sensitivity assay. Top: Expected positions of nucleosomes on permissive 603-P template before (Nucl.) and after ISW2 remodeling (Nucl*ISW2). Unique sites for restriction enzymes are indicated. Bottom: Analysis of end-labeled 603-P nucleosomes by native PAGE before and after remodeling and digestion with a restriction enzyme. Histone-free DNA was added to the nucleosomal templates as an internal control for restriction enzyme activity. (C) Mapping of 603-P nucleosome positions before and after remodeling by ISW2: DNase I footprinting. ISW2 induces translocation of the 603-P nucleosome by 36 bp. Footprinting of DNA (D) and the nucleosome (N) was conducted before and after remodeling. Analysis by denaturing PAGE. Nucleosome positions are indicated (dashed lines and ovals).