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. 2011 Jan 17;39(9):3520–3528. doi: 10.1093/nar/gkq1273

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Published by Oxford University Press 2011.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — The 603-nucleosome positioning sequence does not form a polar barrier to transcription in vivo. (A) Yeast strains with 603 inserted at +56 in the 5′-untranslated region of CUP1. The major transcription start site (+1) is indicated by the arrow. The 603-NP and 603-P strains have 603 inserted in the non-permissive or permissive orientations, respectively. (B) Time courses of induction with 50 µM CuSO₄. The phosphorimager scan of a northern blot probed for CUP1 and ACT1 transcripts is shown. (C) Time courses of induction: WT (circles); 603-P (triangles); 603-NP (squares). The data represent the means of two independent experiments, with standard deviations.