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. 2011 Jan 17;39(9):3520–3528. doi: 10.1093/nar/gkq1273

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Published by Oxford University Press 2011.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Evidence for nucleosomes positioned over the CUP1-603 junction sequences in non-induced cells. Detection of nucleosomes positioned over the CUP1-603 junctions in (A) 603-NP cells and (B) 603-P cells. The schematics show a nucleosome correctly positioned on 603. The dotted ovals represent nucleosomes in positions overlapping the junctions of 603 with CUP1. The locations of the upstream and downstream CUP1 primers and of internal 603 primers 1 and 2 are indicated (small arrows). Phosphorimages of PCR assays are shown. NCP: nucleosome core particles; gen. DNA: genomic DNA; M: marker (75 and 100 bp). Lanes 1–6: multiplex PCR with the upstream junction primer pair and PHO5 primers (internal control). Lanes 7–12: PCR with the downstream junction primer pair and PHO5 primers. Similar results were obtained with copper-induced cells (Supplementary Figure S5). The data are quantified in Supplementary Table S2.