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. 2011 Jan 18;39(9):3543–3557. doi: 10.1093/nar/gkq1297

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Colocalization of BEAF and ME on polytene chromosomes. Transgenic larvae carrying ME on the third chromosome were dissected and used for immunoFISH. (a) Probe ME is in red, anti BEAF antibody is in green. Clear colocalization can be seen both on third and fourth chromosome (white arrowheads). (b) BEAF does not colocalize to the transgene in case of flipped out line. Probe lacZ is in red, anti BEAF antibody is in green. (c) Colocalization of GAF and ME at the site of transgene insertion probed by lacZ on polytene chromosomes. Probe lacZ is in red, GAF antibody signal is in green. Clear colocalization can be seen (white arrowhead). (d) GAF does not colocalize to the transgene in case of flipped out line.