Figure 1.

Dcr-2 interacts with the C-terminal 22 amino acids specific to Loqs-PD. (A) Top panel: Co-immunoprecipitation of endogenous Dcr-2 with GFP-fusion constructs in S2-cells; bottom panel: α-GFP western blot to determine expression levels and successful immunoprecipitation. Fusion of the Loqs-PC specific amino acids to GFP reduce the expression levels; this appears to be generally the case with GFP-PC constructs (see also Figure 2A). (B) Effect of Loqs isoform overexpression on GFP expression levels of the endo-siRNA cell culture reporter; protein isoforms are depicted on the right (dsRBDs are represented by symbols; Loqs-PD-specific sequence is colored in grey); measurement values represent the mean ± SD (n = 3) and were normalized to a pUC18 transfected control (dashed line).