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. 2011 Jan 12;39(9):3632–3642. doi: 10.1093/nar/gkq1337

Figure 1.

Figure 1.

ING1b KD sensitizes cells to UV at S phase. (A) HCT116 cells were transfected with control or ING1b siRNA and harvested for western blot (WB) at various time points. (B) ING1b KD sensitizes cells to UV. HCT116 cells were transfected with control or ING1b siRNA, irradiated with 10 J/m2 UVC, and harvested at indicated times after UV for PI staining followed by FACS analysis. Data was shown as mean ± SEM from three independent experiments (*P < 0.05). (C) Cell cycle analysis for ING1b KD cells. Control or ING1b KD HCT116 cells were arrested at G1 by serum starvation, irradiated with 10 J/m2 UVC and released in the presence of 50 ng/ml nocodazole followed by cell cycle analysis. (D) ING1b KD cells fail to recover from stalled replication. Control or ING1b KD HCT116 cells were pulsed with 20 μM BrdU, irradiated with 10 J/m2 UVC and chased for 12 h. Cells were stained with anti-BrdU antibody and PI, and analysed by FACS. (E) Quantification of BrdU-labelled cells in G2/M phase from three independent experiments (*P < 0.05).