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The blockade on the induction of oral tolerance induced by CT and LT lasts for at least 6 months. (A) Table displaying immunization strategy. (B) Groups of mice were immunized as outlined in panel A and detailed in Materials and Methods. Blood was collected from the tail vein and serum was isolated from individual mice. Endpoint titers were determined by serial dilutions by using IgG ELISA with OVA-coated plates. Endpoint titers of OVA-specific IgG in total serum are shown.
