FIG. 3.

Enzymatic activity is required for CLETS to induce a blockade on the induction of oral tolerance. (A) Table displaying immunization strategy. (B) Groups of five mice were immunized as shown in panel A and detailed in Materials and Methods. Blood was collected from the tail vein, and serum was isolated from individual mice. Endpoint titers were determined by serial dilutions by using IgG ELISA with OVA-coated plates. Endpoint titers of OVA-specific IgG in total serum are shown. The values shown are representative of the results of two experiments.