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. 2011 Feb 9;39(9):e60. doi: 10.1093/nar/gkr063

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — EdU incorporation in fission yeast strains expressing TK and hENT1. (A) Wild-type (P2), TK (adh1:tk, P2471) and TK hENT1 (adh1:tk adh1:hENT1, P2470) expressing strains were grown in YE3S containing 10 µM EdU for 3 h then fixed and conjugated to Alexa Fluor 488 azide before analysis by flow cytometry, (B) adh1:tk (P2471) cells were grown in YE3S containing the indicated concentrations of EdU for 3 h then analysed as in (A), (C) Cells from (A) and (B) were imaged by fluorescence microscopy. Bar = 10 µm and (D) adh1:tk adh1:hENT1 (P2470) cells were grown in YE3S containing the indicated concentrations of EdU for 3 h then processed as in (A) and analysed by flow cytometry. Linear x-axes are used to show EdU incorporation in all panels in this figure.