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. 2011 Feb 11;39(9):e63. doi: 10.1093/nar/gkr065

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Variation of reference gene UBC calculated as the standard deviation of the log transformed gene expression levels after normalization with the arithmetic mean Cq value of three other reference genes (HMBS, SDHA, HPRT1). Variation is measured in increasing numbers of both best quality samples (blue curve) and worst quality samples (red curves) starting with 50 samples up to all 615 samples (quality here defined by 5′–3′ dCq value). The null distribution was determined upon 100 random permutations (grey curves denote 90% distribution range). Similar results were obtained for the other reference genes and RNA quality parameters.