FIGURE 2.

Effect of Dennd4C overexpression on cell surface GLUT4. A, 3T3-L1 adipocytes were transfected with the plasmids for HA-GLUT4-GFP and vector (Vec), wild-type Dennd4C (WT), or the Dennd4C S1043A/S1096A/S1321A mutant (3S/A), and cell surface GLUT4 was measured. The values are the mean ± S.E. from two separate experiments. B, samples from the experiments in panel A were immunoblotted for Dennd4C (D4C). The 1× load was 30 μg of protein. A replicate with the second set of samples gave similar results. Based upon quantification of the immunoblots, the increases in the total amount of Dennd4C averaged 1.9- and 2.2-fold upon transfection with wild-type and S1043A/S1096A/S1321A Dennd4C, respectively.