TABLE 3.

Summary of HTS quality control parameters for 96-well plates

Parameter	Accumulation		Degradation
	UbG76V-GFP reporter	ODD-Luc reporter	UbG76V-GFP reporter	ODD-Luc reporter
S/Ba	3.4	11	2.6	5.8
CVb	3.3% (P), 1.9% (N)	7.3% (P), 4.5% (N)	7.4% (P), 7.3% (N)	8.2% (P), 7.6% (N)
Z′-factorc	0.67	0.7	0.5	0.57

^a S/B, signal/base line ratio.

^b CV, coefficient of variation ((S.D./mean) × 100).

^c Z′-factor = 1 − ((3 × (σp + σN))/(μP − μN)), where σ is S.D. and μ is the mean for positive (P) and negative (N) controls. The positive control was MG132 (2 μm), and the negative control was DMSO (0.8%).