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Oleate attentuates palmitate-induced ceramide production. Mouse C2C12 myotubes were treated with vehicle control, 1.25 mm palmitate, 1.25 mm palmitate plus 0.75 mm oleate, or 0.75 mm oleate alone. Lipid profiles were determined by LC/MS after 16 h of treatment. A, total dihydroceramide and ceramide. B, N-acyl chain length of ceramides measured. Data are means ± S.E. (n = 2) of three experiments. CTL, control; PAL, palmitate; OLE, oleate; CER, ceramide; DHCer, dihydroceramide.
