FIGURE 3.

Fatty acid specificity of DES1 message regulation. A, C2C12 myotubes were treated with vehicle control, 1.25 mm palmitate, or 1.25 mm stearate for 16 h. DES1 mRNA expression was determined by qPCR. Data are presented as means (n = 3) ± S.E. B, C2C12 myotubes were treated with vehicle control, 1.25 mm palmitate, or 1.25 mm palmitate plus 0.3 mm C16:1, C18:1, C20:1, and C18:2. At 16 h of treatment, DES1 mRNA expression was determined by qPCR. Data are presented as means (n = 3) ± S.E. CTL, control; PAL, palmitate.