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. 2011 Mar 24;286(19):16606–16614. doi: 10.1074/jbc.M111.227611

FIGURE 2.

FIGURE 2.

E2F1 and E2F5 3′-UTRs are targets of miR-205. A, the 3′-UTR of E2F1 or E2F5 and mutant 3′-UTR sequences that abolished binding. B and C, luciferase assay showing decreases in reporter activity after co-transfection of either E2F1–3′-UTR or E2F5–3′-UTR with miR-205 in C8161.9 cells. The mutant UTRs of either E2F1 or E2F5 had no effect on reporter activity. D and E, relative mRNA expression levels of E2F1 and E2F5 after transfection with miR-205 or negative control pre-miR. Each experiment was performed in triplicate. *, p < 0.05. Cont.miR, negative control pre-miR.