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. 2011 Mar 24;286(19):16606–16614. doi: 10.1074/jbc.M111.227611

FIGURE 3.

FIGURE 3.

miR-205 suppresses E2F1 and E2F5 expression at the protein level and regulates the AKT pathway. A, relative miR-205 expression level in C8161.9 cells after transfection with miR-205 as determined by miR qRT-PCR. B, Western blot analysis showing decreases in the phosphorylation levels of AKT, BAD, and caspase-9 in C8161.9 cells after miR-205 transfection. C, Western blot analysis showing up-regulation in the phosphorylation levels of AKT, BAD, and caspase-9 after E2F1 overexpression in C8161.9 cells. Cont.miR, negative control pre-miR.