FIGURE 4.

miR-205 inhibits melanoma cell proliferation and colony formation, and induces apoptosis. A, the proliferative ability of C8161.9 cells after miR-205 transfection is reduced significantly as compared with negative control pre-miR (Cont.miR). B, miR-205 overexpression significantly inhibits the colony formation ability of melanoma cells. C, cell cycle analysis showing increase in the sub-G₁ phase of C8161.9 cells overexpressing miR-205. D, Western blot analysis showing increases in the cleavage of caspase-3 and PARP and increased release of cytochrome c in miR-205-transfected cells. *, p < 0.05 (experiments depicted in A–C performed in triplicate).