FIGURE 5.

Stable overexpression of miR-205 inhibits cell proliferation in vitro and in vivo. A, relative miR-205 expression levels in C8161.9 cells stably expressing miR-205 as determined by miR qRT-PCR. B, stable overexpression of miR-205 in melanoma cell lines significantly suppressed cell proliferation. C, Western blot analysis showing suppression in E2F1, E2F5, and AKT phosphorylation levels. D, colony formation ability is significantly reduced by miR-205. E, tumor volume following subcutaneous injection of C8161.9 cells expressing miR-205 was reduced significantly (n = 10 mice per group). Western blot showing expression of E2F1 and E2F5 from tumors from pEP-Null and pEP-miR-205 mice.*, p < 0.05.