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. 2011 Mar 16;286(19):16615–16622. doi: 10.1074/jbc.M111.223784

FIGURE 2.

FIGURE 2.

R164Q mutation disrupted the interaction of PKR2-IL2 to Gα-protein. A, GST or GST fusion proteins with IL2 of WT, H159Q, and R164Q-PKR2 were used to pulldown Gαq-protein. B, GST or GST fusion proteins with IL2 of PKR2 were used to pulldown WT or truncated Gαq-protein without the C-terminal five amino acids (Gαq-ΔC5). C, GST or GST fusion proteins with IL2 of WT and R164Q-PKR2 were used to pulldown Gαqi5-protein. The proteins were analyzed by Western blotting with anti-HA antibody to detect the HA tag at the N terminus of Gα-proteins.