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Effect of IGF-I on VSMC alkaline phosphatase expression and mineral deposition by VSMCs. Cells were maintained in growth medium with or without IGF-I (5–100 ng/ml) for 24 h and then stimulated with β-GP (5 mm) for 4 days (alkaline phosphatase (Alk Phos); A and B) or for 8 days (mineralization; C and D). Alkaline phosphatase activity and calcium deposition were determined. Results are the mean ± S.D. of three experiments performed in triplicate.
