FIGURE 7.

Effect of IGF-I and N-linked glycosylation inhibitors on VSMC mineralization. Cells were maintained in growth medium with or without des-1–3-IGF-I (25 ng/ml) and glycosylation inhibitor for 24 h and then stimulated with β-GP (5 mm) for 8 days. Cells were washed, deposited calcium was dissolved in 0.6 n HCl, and the calcium content was determined by the O-cresolphthalein method. Mineralization is expressed as milligrams of calcium/mg of total cellular protein and is the mean ± S.D. of three experiments performed in triplicate.