FIGURE 7.

007 activates Rap1 and decreases RhoA activity in SMCs. A, a Western blot shows 007-induced increased Rap1 and decreased RhoA activity for serum-starved rat aortic, human airway, and mouse fundus SMCs. B, LPA (1 μm) significantly increased RhoA activity in all three cell types (p < 0.01, p < 0.003, and p < 0.001, respectively) that were reduced by treatment with 007 (50 μm) (p < 0.007 n = 3, p < 0.04 n = 3, p < 0.01, respectively). A, C, and D, 007 significantly increased Rap1 activity in rat aortic (n = 3), human airway (n = 3), and fundus (n = 8) SMCs compared with control cells (p < 0.03 (*), p < 0.001(#), and p < 0.02 (+) respectively). B, statistical significance for each comparison designated by brackets was p < 0.05.