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EPR spectrum of the ferric heme-bound frog DGCR8 HBD-His6 protein. The frog HBD-His6 protein (153 μm) was in 50 mm EPPS (pH 8.0) and 400 mm NaCl. The spectrum represents an average of 10 scans taken at 10 K, with 9.383 GHz microwave frequency, 8.000 G modulation amplitude, 100 kHz modulation frequency, 60 dB receiver gain, 163.84 ms time constant, and a power of 1.002 milliwatt. The asterisk represents a signal present in the cavity.
