FIGURE 4.

Functional redundancy between NuB4 complex components, histone H3, and Asf1p in DNA repair-linked chromatin reassembly. A, 10-fold serial dilutions of the indicated strains were spotted on plates containing either glucose or galactose as carbon source. Plates were incubated at 30 °C for 3 days and then photographed. B, cutting and repair of the MAT locus in the indicated strains was monitored as described in the legend to Fig. 2. B, the levels of MATα and MATa fragments were quantitated as described in the legend to Fig. 2 except that the MATa levels are plotted relative to the 6-h time point. D, ChIP analysis of H3 levels near the MAT locus at the indicated times. Galactose was added to cultures at time 0. Glucose was added after 3 h. ChIP experiments were performed and analyzed as described in the legend to Fig. 2.