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Characterization of EXLX1 wall extension activity (creep) with alkali-pretreated wheat coleoptiles. A, concentration dependence. B, pH dependence using 150 μg/ml EXLX1. The buffers used to stabilize the pH were 25 mm NaOAc, pH 4.5; 25 mm NaOAc, pH 5.5; 25 mm HEPES, pH 7.5; 25 mm Tris, pH 9.5; 25 mm borate, pH 10.5; 25 mm borate, pH 11.5; 25 mm sodium phosphate buffer, pH 10.5; and 25 mm sodium phosphate buffer, pH 11.5. Error bars indicate S.E. (4 ≤ n ≤ 20).
