FIGURE 6.

Binding of EXLX1 to wheat coleoptile cell walls and cellulose. A, binding isotherm of EXLX1 to cell walls. HEPES (25 mm), pH 7.5 was used as buffer. The effect of CaCl₂ on EXLX1 binding to wheat coleoptile cell walls (B) and Avicel (C) is shown. In both cases, 12 μg/ml EXLX1 was incubated in 0.3 ml of 25 mm HEPES, pH 7.5 containing 0.5 mg/ml cell walls or 10 mg/ml Avicel. Error bars indicate S.E. (3 ≤ n ≤ 6). D, binding of EXLX1 to cell wall residues after sequential extraction of matrix polysaccharides. E, binding isotherm of EXLX1 to Avicel. HEPES (25 mm), pH 7.5 was used as buffer. Error bars indicate S.E. (4 ≤ n ≤ 10).