FIGURE 2.

VGSC activity in HUVECs. A, electrophysiological whole-cell recordings. Traces show activation of an inward current by applying 5-mV steps, from −70 mV to +70 mV, from a holding potential of −100 mV, with an interpulse interval of 2 s. Alternate traces only are shown for clarity. B, a typical current-voltage relationship for the inward currents recorded as in A. C, TTX dose-response curve. Data points denote means ± S.E. (error bars) (n ≥ 4). Cells were pulsed from a holding potential of −100 mV to −10 mV for 30 ms with a repeat interval of 10 s. The effect of TTX was recorded on the fifth pulse. The intracellular pipette solution contained Cs⁺ to block outward (K⁺) currents in all recordings shown.