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. 2011 Mar 8;286(19):16846–16860. doi: 10.1074/jbc.M110.187559

FIGURE 4.

FIGURE 4.

Effect of VGSC (Nav1.5 and Nav1.7) siRNAs on chemotaxis. mRNA measurements following treatments of HUVECs with Nav1.5 (A) and Nav1.7 (B) siRNA. Normalized levels of mRNA at 72 h relative to cells transfected with non-targeting siRNA are shown. White bars, Nav1.5 siRNA-transfected HUVECs. Gray bars, Nav1.7 siRNA-transfected HUVECs. Bars, mean ± S.E. from 2−ΔΔCT analysis (n = 3). C, representative Western blots of proteins from siRNA-treated HUVECs using a pan-VGSC primary antibody and GAPDH. Un, untreated; M, mock-transfected; other lane labels are as in A. D, Transwell chemotaxis of HUVECs treated with Nav1.5 or Nav1.7 siRNA. Data are shown as percentage controls. Samples are as in A but also showing the effect of TTX applied post-transfection. Each bar denotes mean ± S.E. (error bars) (n = 3). *, p < 0.05.