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. 2011 Mar 8;286(19):16846–16860. doi: 10.1074/jbc.M110.187559

FIGURE 5.

FIGURE 5.

TTX inhibits ERK1/2 activation upon VEGF stimulation of HUVEC. HUVECs were incubated with VEGF in the presence of TTX for the indicated times. A, Western blots of phospho-ERK1/2, total ERK1/2, and GAPDH (n = 4). B, phospho-PLCγ1, total PLCγ, p-ERK/12, and total ERK levels (n = 3). C, HUVECs were incubated with EGF in the presence of TTX as indicated, and blots were probed as in A (n = 3). D, HUVECs were stimulated with VEGF in a “low Na+” medium; ERK1/2 and PLCγ1 activation were analyzed as before (n = 3). E, representative Western blot of phosphorylated and total ERK1/2 from HUVECs transfected with siRNA (100 nm), serum-starved and challenged with VEGF (50 ng/ml for 10 min). Un, untreated; M, mock-transfected; 1.7, Nav1.7 siRNA; 1.5, Nav1.5 siRNA.