Skip to main content
. 2011 Mar 7;286(19):16879–16890. doi: 10.1074/jbc.M110.186932

FIGURE 1.

FIGURE 1.

Inhibition of GSK-3β induces Ca2+-independent deposition of junction components. A, confluent MDCK cells were incubated in LCM for 16 h and then switched back to HCM for the indicated times. Cell lysates were probed with the indicated antibodies in a Western blot analysis. B, confluent MDCK cells were treated with DMSO, 10 μm SB216763, 20 mm NaCl, or 20 mm LiCl for 2 h. Cell lysates were probed with the indicated antibodies in a Western blot analysis. C, confluent MDCK cells were incubated in LCM for 16 h followed by replacement of the media with fresh LCM supplemented with DMSO, 10 μm SB216763, 20 mm NaCl, or 20 mm LiCl. Cells were fixed at the indicated time points after the replacement of medium and immunostained for ZO-1. Bar, 30 μm. D, quantification of ZO-1 relocalization to cell membrane in C. Data are representative of three independent experiments (n = 3). Error bars represent means ± S.D. ZO-1 length per cell is within each of the four randomly picked view fields. The asterisks denote significant difference in the indicated pairs (p < 0.05) by Student's t test. E, MDCK cells were treated as described in C, except that they were instead immunostained for occludin. Bar, 30 μm. F, quantification of occludin relocalization to cell membrane in E. Error bars represent means ± S.D. for occludin length per cell within each of the four randomly picked view fields. The asterisks denote significant difference in the indicated pairs (p < 0.05) by Student's t test. G, confluent MDCK cells were incubated in LCM for 16 h followed by replacement of the media with fresh LCM supplemented with the indicated concentrations of different test reagents. Cells were fixed 2 and 10 h after the medium change and immunostained for ZO-1. Bar, 30 μm. H, quantification of ZO-1 relocalization to cell membrane in G. Data are representative of two independent experiments (n = 2). Error bars represent means ± S.D. for ZO-1 length per cell within each of the four randomly picked view fields. The asterisks denote significant differences in the indicated pairs (p < 0.05) by Student's t test.