TABLE 1.

The effects of the solvent isotope on the kinetics of the P2O oxidative half-reaction

The reactions were performed in 100 mm sodium phosphate pH(D) 7.0, at 4 °C in a stopped-flow spectrophotometer.

Parameters	H2O buffer	D2O buffer
k1app (m−1 s−1)	5.6 ± 0.2 × 104	5.9 ± 0.3 × 104
k1sim (m−1 s−1)	6.5 ± 0.3 × 104	6.5 ± 0.3 × 104
k−1sim (s−1)	2 ± 0.1	2 ± 0.1
k2app (s−1)	24 ± 2	6.9 ± 0.5
k2sim (s−1)	18 ± 1	6.4 ± 0.3