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. 2011 Mar 24;286(19):16929–16939. doi: 10.1074/jbc.M110.215871

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Model for the formation of the ERAD complex containing HRD1-SEL1L in the ER membrane and ERAD regulation. The large complex containing HRD1-SEL1L (Complex I) regulates the ERAD of glycosylated NHK and non-glycosylated NHK-QQQ. By the transient expression of HRD1-SEL1L, a smaller-sized complex (Complex II) was formed in which OS-9 associates at the luminal side. Complex II was also capable of extraction and degradation of both NHK and NHK-QQQ. SEL1L that does not associate with HRD1 is rapidly degraded by an E3 other than HRD1. NHK-QQQ interacting with SEL1L was also rapidly degraded through the function of this E3. Destabilization of SEL1L by the silencing of HRD1 as well as depletion of SEL1L by siRNA treatment targeted for SEL1L resulted in the release of OS-9 from the membrane complex. Only the molecules analyzed in this study are shown. O/E, overexpression; K/D, knockdown.