FIGURE 3.
KCa3.1 protein expressions in thymus, spleen, and lymph node (mesenteric) of humans (A) and mice (B). Protein fractions from cytosol (C) and plasma membrane (M) were probed by immunoblotting with anti-KCa3.1-C antibody. Molecular mass standards are shown in kilodaltons on the left side of the panels. C, co-immunoprecipitation of KCa3.1b with KCa3.1a. C, panel a, plasma membrane protein fraction of mouse thymus was probed by immunoblotting (IB) with anti-KCa3.1-C antibody, which recognized the N-terminal region of KCa3.1 (anti-KCa3.1-N) (left lane). Right lane shows the membrane blotted with KCa3.1-N with excess antigen. C, panel b, mouse thymus protein lysate was immunoprecipitated with anti-KCa3.1-N and probed by immunoblotting with anti-KCa3.1-C, which recognized the C terminus of KCa3.1. Arrowheads indicate the migrating positions of KCa3.1a (50 kDa) and KCa3.1b (25 kDa). Molecular mass standards are shown in kilodaltons on the left side of the panels.