FIGURE 2.
Expression of Ucp1, Dio2, and Fabp4 in wild-type and Sirt3-null brown adipocytes and Sirt3-null MEF-derived adipocytes. The tops of panels A and B show micrographs representative of differentiated primary cultured brown adipocytes and MEF-derived adipocytes, respectively, from wild-type and Sirt3-null mice. Bars are mean ± S.E. of four to five independent cultures (*, p < 0.05, NE or cAMP versus controls; #, p < 0.05, wild-type versus Sirt3-null cells). NE, 0.5 μm norepinephrine for 6 h; cAMP, 1 mm dibutyryl-cAMP for 24 h. c, control.