FIGURE 4.

The ribonuclease H activities of RNase H2A mutants. Reactions containing RNase H2A WT (lane 2, 0.4 nm; lane 3, 4 nm; lane 4, 40 nm; lane 5, 400 nm), RNase H2A G37S (lane 7, 0.4 nm; lane 8, 4 nm; lane 9, 40 nm; lane 10, 400 nm), RNase H2A R186W (lane 12, 0.4 nm; lane 13, 4 nm; lane 14, 40 nm; lane 15, 400 nm), or RNase H2A R235Q (lane 17, 0.4 nm; lane 18, 4 nm; lane 19, 40 nm; lane 20, 400 nm) were incubated with the 1-ribo RNA-DNA (200 nm). Lanes 1, 6, 11, and 16 contain no RNase H2. Reaction products were visualized by denaturing PAGE and quantified as described under “Experimental Procedures.”