TABLE 2.

Parameters derived from SAXS experiments

Sample	Concentrationa	Q rangeb	Rgc	Dmaxc	I(0)c	χ2d
	mg/ml		Å	Å	a.u.
ASV-IN(1–286)e	2.3	0.007–0.4	33.1 ± 0.6	109.4 ± 1.3	0.033	0.8
(wild type)
ASV-IN(1–286)e,f	2.4	0.01–0.4	36.8 ± 0.2	119.7 ± 0.8	0.034	3.4
(E157C/F199K)
ASV-IN(1–286)e	0.7	0.01–0.4	31.5 ± 0.2	92.8 ± 1.0	0.024	1.7
(C23S/C125S/F199K/W259A)
trxA-ASV-IN(1–286)e	2.3	0.01–0.4	39.0 ± 0.4	114.9 ± 1.1	0.030	1.1
(C23S/C125S/F199K/W259A)
ASV-IN(49–286)e,f	1.1	0.01–0.42	26.0 ± 0.8	75.0 ± 2.2	0.022	2.2
(F199K)
ASV-IN(1–207)e,f	3.6	0.01–0.41	20.0 ± 0.1	61.0 ± 0.4	0.011	0.8
PFV-IN(1–402)d	4.3	0.007–0.42	38.5 ± 0.2	117.9 ± 0.6	0.031	2.7
(wild type)

^a Data were determined by absorbance at 280 nm with a calculated molar extinction coefficient.

^b Q = 4πsinθ/λ, where 2θ is the scattering angle; recorded data in this range were used for P(r) analysis and subsequent ab initio shape reconstructions.

^c This was determined using the program IRENA. Comparable results were obtained using the program GNOM and by Guinier analysis with Auto R_g (65).

^d Goodness of fit was assessed by reduced χ² analysis.

^e Data were collected at APS beamline DND-CAT 5ID-D.

^f Data were also collected at local sources.