Interactions among PAD4, ING4, and p53. A, shown is a schematic diagram of MBP-p53 and its truncations used in the pulldown experiments. B, the RD of p53 is necessary and sufficient for the interaction with ING4. MBP-tagged domain deletions of p53 were expressed, purified, and bound to glutathione resin. GST pulldown assays were performed as in Fig. 2D. WB, Western blot. C, shown is a schematic of domain interactions among PAD4, ING4, and p53.