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Direct binding of MCV LT to hVam6p. MBP-hVam6p-bound amylose resin beads were incubated with eluted GST, GST-LT.W209A, or GST-LT proteins for a pull-down assay. 5% input and bound proteins were separated on an 8% SDS-PAGE gel. Immunoblotting (IB) was performed with anti-MCV LT CM2B4 antibody (A) and anti-MBP antibody (B).
